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22.01.2011 11:07:02. time agoExpression of E-cadherin, CD24, and GATA-4 in mouse testes at different stages of sexual maturation.
The expression of E-cadherin (CDH1), a member of the cadherin family expressed in testis and testicular cells, and related to germ cell maturation, was studied in mice at different stages of sexual development: adult (A); prepubertal (P1); and sexually mature (P2). The expression of CD24, a well-known marker of germ cells, and GATA-4, a transcription factor involved in cell differentiation, were also investigated. Immunohistochemistry revealed that E-cadherin was expressed in the basement membrane surrounding the tubules of A and P1 mice testes. Moreover, E-cadherin was expressed in Sertoli cells and in spermatogonia/spermatocytes. In the P2 testis, E-cadherin was still present in both Sertoli cells and in germ cells. The expression of CD24 was first detected in the A testis and was detected in spermatogonia/spermatocytes. In the P1 and P2 mice testes, immunostaining was restricted to Sertoli cells and spermatogonia/spermatocytes. The GATA-4 protein was expressed in A and P2 testis and was detected in spermatogonia/spermatocytes. Our results indicate that the pattern of expression of E-cadherin is conserved in mouse Sertoli cells during sexual maturation. Moreover, we showed that the expression of E-cadherin, CD24 and GATA-4 are all present in germ cells during the early stages of sexual maturation, but it is only in the mature testis that these three factors are still present in germ cells.Persepolis Project
The Persepolis Project was a collaborative archaeological site excavated from 2004 to 2008 in the ancient Persian city of Persepolis. It revealed that this administrative center was destroyed in 514 BC and rebuilt in around 500 BC, when was created by Darius I of Persia.
The Project
The Persepolis Project is a collaborative study group based at the University of Cambridge Museum of Archaeology and Anthropology, University of Oxford and the Ashmolean Museum in
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