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Combating the worldwide problem of antimicrobial resistance is a priority and the World Health Organization (WHO) calls for the development of new treatment options and vaccines ([@bib14]). Among the possible strategies for combating infectious diseases, phage therapy is a promising therapeutic option as it directly targets the pathogen ([@bib22]). As opposed to traditional antibiotics, phages are considered safe and effective: they can be produced at low cost, they are easily isolated from natural sources, and they are not affected by the emergence of drug resistance. Moreover, they are powerful antimicrobial agents as they specifically target and lyse a single bacterium, thereby limiting the possibility of cross-infection to neighboring hosts ([@bib21]).
As the number of bacterial pathogens that produce a strongly adherent biofilm (slime layer) increases, the efficacy of phage therapy is reduced. In the second stage of biofilm formation, bacterial aggregates are produced and surrounded by a multilayered matrix of hydrated, extracellular polymeric substances (EPS) that is mainly composed of polysaccharides, proteins and DNA ([@bib11]). The formation of these biofilms protects the bacteria from the action of the lytic phages, and phages are therefore unable to penetrate the biofilm and lyse their host ([@bib9]).
Matrix components interfere with the efficiency of phages and the drug interaction with host cells in a particular matrix may differ significantly from the interaction in the free-living state. As we have previously shown ([@bib30]), the dense chemical composition of the biofilms significantly alters the specificity and affinity of the interaction between phages and their targets. However, the exact mechanisms of this modulation remain poorly understood. Among the
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This game is a strategy game with RPG elements. It is an ARPG that is based on the Youkai Watch series and features the anti-youkai. The game allows the player to control a team of soldiers and explore a. We have the codes for Swords and Soldiers HD for both Windows.
This game is a strategy game with RPG elements. It is an ARPG that is based on the Youkai Watch series and features the anti-youkai. The game allows the player to control a team of soldiers and explore a. We have the codes for Swords and Soldiers HD for both Windows.
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4 days ago · New Xbox Game Pass releases include Batman: The Telltale Series,. Mega Man 11, and Swords & Soldiers.
If you search for “Xbox Live Gamertag” or “Xbox Live Gamertag” on YouTube, you will not find. The WORTH quest, which was added on August 1st, 2014 unlocks this ability… We have the codes for Swords and Soldiers HD for both Windows.
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