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Free radicals, also called reactive oxygen species, play important roles in lipid peroxidation, cell damage and the development of diabetes. But the production of reactive oxygen species in diabetes is still unclear. We investigated the free radical production in streptozotocin-induced diabetic rats. Male albino Wistar rats were randomized into two groups: control (n = 22) and diabetic (n = 22). Diabetes was induced in the rats by a single intraperitoneal injection of streptozotocin (55 mg/kg). At the end of the experiment, the plasma malondialdehyde (MDA) and protein carbonyl content (PCC) were significantly higher in diabetic than in the control group (p = 0.02 and p = 0.0004, respectively). The plasma PCC and the formation of 4-hydroxy-2-nonenal (HNE) adducts in erythrocytes, was significantly higher in diabetic than in the control rats (p = 0.0049 and p = 0.0007, respectively). The activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were significantly lower in the plasma of diabetic rats than in the control group (
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